The resulting first strand cDNA was purified using AMPure XP beads before PCR amplification. The cDNA was added to a master mix of 1× HiFi Kapa PCR mix, 0.5 μM of forward and reverse primer ...

Briefly, a total of 12.5 μl 2× Taq Master Mix were mixed with the specific primer pair for the individual genes, 5 μl cDNA of each sample used as ... Software, data curation, writing—original draft ...

The 2X PCR Master Mix is a made-to-order custom master mix. Once your order is received, your custom master mix will be delivered in 10 business days. The 2X formulation contains customer defined ...