Most vertebrate cells in culture only grow and divide for a limited ... and acquire the ability to divide and grow indefinitely. HeLa cells are unique in many ways. The normal human cells contain ...

Regarded from the perspective of expanding the knowledge base, there can be little doubt that the discovery and exploitation ...

four times by passage of uninoculated HeLa-cell culture fluids to fresh HeLa cell and monkey-kidney cultures, and twice from infants with respiratory illnesses. Hypotheses concerning the origin of ...

In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...

Her cells, known as "HeLa cells," have since been sent around the world, and have led to numerous medical breakthroughs. A movie about her story was made in 2017, starring Orpah Winfrey.

The HeLa Kyoto cell line and derived stable cell line GFP-Nup107 (purchased ... A11138-03)] or the vehicles (DMSO 1:1,000 and HEPES KOH 200 nM) in culture media for 7 h at 37°C. To induce the ...

Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media ... After reaching 50–60% confluence, the cells were transitioned to serum-free culture medium and allowed to grow ...

The cancer-associated fibroblasts (CAFs) and HeLa cell line were obtained from lab freezing ... For the WST-1 assay, the cell culture medium was replaced with fresh medium containing 10% WST-1. After ...

The British embryologist’s work has been consistently overlooked in our male-dominated scientific ecosystem – but her story ...

The different cell lines were maintained as monolayers in plastic culture flasks (25 cm 2) in culture medium (HAM-F10 + DMEM, 1:1, Sigma–Aldrich or only DMEM) supplemented with 10% fetal bovine serum ...