A new technology developed at MIT enables scientists to label proteins across millions of individual cells in fully intact 3D ...

Enter SynPull, a new synaptosome imaging method developed by David Klenerman, John Danial, and colleagues at the University of Cambridge, England. SynPull combines single-molecule pull-down of ...

Images were taken by electron microscopy (JEM-1400) (Liu et al., 2017). ESCs were cultured on gelatin-coated glass slides, and mitochondria were ... incubated with primary antibodies for 14 h at 4°C, ...

followed by incubation with Alexa Fluor 488-conjugated secondary antibody (Beyotime Biotechnology, China) for 1 h at room temperature. Slides were stained with DAPI and immunofluorescence images were ...

Monoallelic loss of PTEN and ARID1A is identified in normal colonic epithelium. The resulting haploinsufficient phenotypes ...

Cells navigating in complex microenvironments frequently encounter narrow spaces that physically challenge migration.

The management of Leishmania donova ni (LD), responsible for fatal visceral leishmaniasis (VL), faces increasing challenges due to rising drug-unresponsiveness, leading to increasing treatment ...

This valuable study presents a mouse gastruloid model that can be used to generate hematopoietic progenitors as well as leukemic cells. However, in its current form, the manuscript is inadequate ...

Researchers from the Tanenbaum group at the Hubrecht Institute have developed a new microscopy technique to observe how ribosomes function in cells.

Ideally, the reactants should function similar to an antibody-antigen duo, reacting rapidly with one another, unaided by auxiliary reagents, to form a stable adduct with innocuous (or no ...

Electron microscopy uses a beam of electrons to illuminate a sample and achieve much higher spatial resolution than light microscopy. Transmission electron microscopy generates an image of the ...

Genetic alterations may help mosquitoes adjust to climate change, shifting the distribution of mosquito-borne disease in a warming planet.