The excitation is typically performed using a focused laser beam. The core principle of STED microscopy is the selective deactivation of fluorophores using stimulated emission. A second laser, called ...

The design integrated the underlying principles of STED microscopy and the optical vortex method into a single configuration. Remarkably, the performance of this new design surpassed the ...

Essentially any structure that can be labelled can, in principle, be imaged. Leica Microsystems now sells an STED microscope with a resolution of 80 nm. At present, the far-field methods are those ...

The framework independently discovered a way to integrate the underlying physical principles from the aforementioned SR techniques (STED microscopy and the optical vortex method) into a single ...

SIM is one of several super-resolution microscopy techniques that have been developed to overcome the diffraction limit. Here's a comparison with two other popular techniques: STED microscopy achieves ...