Serial dilutions were performed using DNA extracted from Nras mutation-bearing ... The presence of mutations was verified by comparison with genomic sequences provided by the BLAST NCBI database.

The initial melting temperature of 93 °C should be used only for genomic DNA. For other templates, the use of lower temperatures (80 °C) is advised to avoid depurination. The precise annealing ...

The dye causes the unwinding process to give off fluorescent light, creating what researchers call a melting curve-;a unique signature for each type of pathogen. In the past, DNA melting has ...