Incubate the cultures at 37 °C for 20–24 h in a humidified incubator with an atmosphere of 5–7% CO 2. Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA copr ...

trigger the paracentric genomic rearrangement event upon co-transfection (Figure 1). The introduction of the EML4-ALK mutation in the cell line was confirmed via Sanger sequencing as shown in ...