When they act on a DNA molecule, restriction enzymes produce "blunt" ends when they cut in the middle of the recognition sequence, and they yield "sticky" ends when they cut at the recognition ...

It had become supercoiled circular DNA again. And the EcoRI site had regenerated.” At the time, scientists assumed that all restriction enzymes probably left blunt ends when they cut DNA because the ...

nebulization or shearing), followed by DNA repair and end polishing (blunt end or A overhang) and, finally, platform-specific adaptor ligation. This process typically results in considerable ...